SUMMARY OBJECTIVE: The aim of this study was to evaluate the prevalence of early neonatal sepsis in pregnant women with a positive culture for group B beta-hemolytic Streptococcus in a middle-income city in Southeastern Brazil. METHODS: A retrospective cohort study was conducted, involving singleton low- and high-risk pregnancies in whom group B beta-hemolytic Streptococcus cultures were evaluated between 35 and 37 weeks of gestation using vaginal and anal swabs. A specific medium (Todd-Hewitt) was used for culturing. The pregnant women were divided into two groups based on positive (n==201) and negative (n==420) cultures for group B beta-hemolytic Streptococcus. RESULTS: The maternal colonization rate by group B beta-hemolytic Streptococcus was 32.3%. The prevalence of early neonatal sepsis was 1.0% (2/201) among patients with a positive group B beta-hemolytic Streptococcus culture and 1.9% (8/420) among patients with a negative culture. Among the patients who underwent adequate prophylaxis, crystalline penicillin G was used in 51.9% (54/104), followed by cefazolin in 43.3% (45/104), ampicillin in 3.8% (4/104), and clindamycin in 1.0% (1/104). A model that included prematurity (p==0.001) proved to be an independent risk predictor of early neonatal sepsis [χ2 (1)==15.0, odds ratio: 16.9, 95% confidence interval: 4.7–61.6, p<0.001, Nagelkerke R2==0.157]. CONCLUSION: The prevalence of a positive culture for group B beta-hemolytic Streptococcus was high. However, the prevalence of early neonatal sepsis was low in pregnant women with both positive and negative group B beta-hemolytic Streptococcus cultures and in pregnant women with a positive culture who underwent both adequate and inadequate antibiotic prophylaxis. Prematurity proved to be an independent predictor of early neonatal sepsis, considering the entire study population.

ABSTRACT The aim of this study was to evaluate intake, digestibility and performance in dairy heifers fed diets containing increasing levels of babassu cake (0, 100, 200 and 300 g kg-1 dry matter - DM) as a substitute for sugarcane treated with calcium oxide. Twenty-four heifers with an initial mean weight of 190.8 + 40.8 kg were used, distributed in a completely randomised design. The dry matter intake (DMI), neutral detergent fibre intake (NDFI) and acid detergent fibre intake (ADFI), as %BW and g BW-0.75 , showed an increasing linear response. The organic matter intake (OMI) showed a linear increase of 40 g day-1, 0.017%BW and 0.66 g BW-0.75 for each 10 g kg-1 increase in babassu cake in the diet. The apparent digestibility of the dry matter (ADDM) and organic matter (ADOM) showed a quadratic response (P < 0.05), with a minimum of 493 g kg-1 DM for 150 g kg-1 DM and 526.5 g kg-1 DM for 320 g kg-1 DM as a substitute for the sugarcane, respectively. The final mean weight was not altered, presenting a value of 299.7 + 45.5 kg. Daily weight gain (DWG) showed a quadratic response, with the highest value at a substitution level of 190 g kg-1 DM. The feed conversion rate of the DM was not affected by the addition of babassu cake, with an average of 4.6 + 0.74 kg DM kg-1 in weight gain. Babassu cake can substitute hydrolysed sugarcane in heifer diets up to 300 g kg-1 DM, with the greatest weight gain seen at a substitution level of 190 g kg-1 DM.

Abstract Background The use of the timed up and go (TUG) test to assess cardiorespiratory fitness in patients with heart disease has not been well defined in the literature. Objectives Test the association between TUG and peak oxygen consumption (VO2peak), construct an equation based on TUG to predict VO2peak, and determine a cutoff point to estimate VO2peak ≥ 20 mL/kg/min. Methods This cross-sectional study included 201 patients with coronary artery disease or heart failure, between 36 and 92 years of age, who underwent TUG and cardiopulmonary exercise test. Correlation, ROC curve, multiple linear regression, and Bland-Altman analyses were performed. The significance level was set at p < 0.05. Results The mean age of the total sample was 67 ± 13 years, and 70% of participants were male. The mean VO2peak was 17 ± 6 mL/kg/min, and the mean TUG time was 7 ± 2.5 seconds. The correlation between VO2peak and TUG was r = −0.54 (p < 0.001), and R2 was 0.30. The following equation was developed based on TUG: V O 2 peak = 33.553 + ( − 0.149 × age ) + ( − 0.738 × TUG ) + ( − 2.870 × sex ); a value of 0 was assigned to the male sex and 1 to the female sex (adjusted R: 0.41; adjusted R2: 0.40). The VO2peak estimated by the equation was 18.81 ± 3.2 mL/kg/min, and the VO2peak determined by cardiopulmonary exercise test was 18.18 ± 5.9 mL/kg/min (p > 0.05). The best cutoff point in the TUG for VO2peak ≥ 20 mL/kg/min was ≤ 5.47 seconds (area under the curve: 0.80; 95% confidence interval: 0.74 to 0.86). Conclusions TUG and VO2peak showed a significant association. A prediction equation for VO2peak was developed and validated internally with good performance. The cutoff point in the TUG to predict VO2peak ≥ 20 mL/kg/min was ≤ 5.47 seconds.

Resumo Fundamento A utilização do teste timed up and go (TUG) na avaliação da aptidão cardiorrespiratória em cardiopatas não está bem definida na literatura. Objetivos Testar a associação entre o TUG e o consumo de oxigênio de pico (VO2pico), construir uma equação com base no TUG para prever o VO2pico e determinar um ponto de corte para estimar um VO2pico ≥ 20 mL.kg−1.min−1. Métodos Estudo transversal com 201 indivíduos portadores de doença arterial coronariana ou insuficiência cardíaca, com idade entre 36 e 92 anos, submetidos ao TUG e ao teste cardiopulmonar de exercício. Foram realizadas análises de correlação, curva ROC, regressão linear múltipla e Bland-Altman. Um p < 0,05 foi adotado como significante. Resultados A média de idade da amostra total foi 67 ± 13 anos, e 70% dos participantes eram do sexo masculino. A média de VO2pico foi de 17 ± 6 mL.kg−1.min−1 e a média de desempenho no TUG foi de 7 ± 2,5 segundos. A correlação entre o VO2pico e o TUG foi r = −0,54 (p < 0,001) e R2 de 0,30. Foi desenvolvida a equação com base no TUG: V O 2 pico = 33 , 553 + ( − 0 , 149 ∗ idade ) + ( − 0 , 738 ∗ T U G ) + ( − 2 , 870 ∗ sexo ); sendo atribuído o valor 0 ao sexo masculino e 1 ao sexo feminino (R ajustado: 0,41; R2 ajustado: 0,40). O VO2pico estimado pela equação foi 18,81 ± 3,2 mL.kg−1.min−1 e o determinado pelo teste cardiopulmonar de exercício foi 18,18 ± 5,9 mL.kg−1.min−1 (p > 0,05). O melhor ponto de corte para o VO2pico ≥ 20 mL.kg−1.min−1 foi de ≤ 5,47 segundos (área sob a curva: 0,80; intervalo de confiança de 95%: 0,74 a 0,86). Conclusões O TUG e o VO2pico apresentaram associação significativa. A equação preditiva do VO2pico foi desenvolvida e validada internamente com bom desempenho. O ponto de corte no TUG para prever um VO2pico ≥ 20 mL.kg−1.min−1 foi ≤ 5,47 segundos.

ABSTRACT Pirarucu, Arapaima gigas is a fish species of great social and economic importance in the Amazon region, where they are often farmed in earthen ponds. Intensive cage aquaculture has been growing in Brazil, which could be an alternative means to farm pirarucu, reducing costs and increasing productivity. We evaluated the contribution of natural food organisms in semi-intensive (ponds) and intensive (cages) production systems, and their effect on pirarucu growth and economic performance during the first phase of grow-out. Four ponds (300 m2) and four cages (4.0 m³) were stocked, respectively, with 120 (0.4 fish m-²) and 160 (40 fish m-³) juvenile pirarucu (28.03 ± 6.34 g, 11.75 ± 0.80 cm). The study was conducted for 105 days and fish were fed with commercial feed. Fish growth and plankton intake were evaluated every two weeks. Survival rate, standard length, weight gain and final weight were higher in fish reared in earthen ponds than in cages. Feed conversion of fish kept in ponds was lower (0.96 ± 0.06) than in cages (1.20 ± 0.11). The consumption of natural food organisms was observed, despite artificial feed being fed in both systems. The relative abundance of zooplankton and insects in stomachs was directly proportional to fish weight gain in ponds, and inversely proportional in cages. Higher economic efficiency rate and lower average production cost were calculated for earthen ponds. Our results indicate that the cost-benefit of the first phase grow-out of A. gigas is better in earthen ponds.

RESUMO O pirarucu, Arapaima gigas é um peixe de grande importância econômica e social na Amazônia, onde é comumente produzido em viveiros. A produção de peixes em tanques-rede tem crescido no Brasil, e este sistema poderia ser uma alternativa para a produção do pirarucu, reduzindo custos e melhorando os parâmetros produtivos. Nós avaliamos a contribuição do alimento natural na produção em sistema semi-intensivo (viveiros) e intensivo (tanques-rede) e seus efeitos sobre os aspectos produtivos e econômicos do pirarucu na fase de recria. Quatro viveiros (300 m2) e quatro tanques-rede (4,0 m3) foram estocados, respectivamente, com 120 (0,4 peixes m-2) e 160 (40 peixes m-3) juvenis de pirarucu (28,03 ± 6,34 g e 11,75 ± 0,80 cm). O estudo foi conduzido por 105 dias e os peixes foram alimentados com ração comercial extrusada. Crescimento e ingestão de plâncton pelos peixes foram avaliados quinzenalmente. Sobrevivência, comprimento padrão, ganho de peso e peso final foram maiores nos peixes cultivados em viveiros. A conversão alimentar nos viveiros (0,96 ± 0,06) foi menor que nos tanques-rede (1,20 ± 0,11). Mesmo sendo ofertada ração, os pirarucus consumiram alimento natural nos dois sistemas de produção. A abundância relativa de zooplâncton e insetos nos estômagos foi diretamente proporcional ao ganho de peso nos viveiros, e inversamente proporcional nos tanques-rede. Um índice de eficiência econômica mais alto e custo médio de produção mais baixo foram calculados para os viveiros escavados. Nossos resultados indicam que o custo-benefício do cultivo de A. gigas durante a fase de recria é melhor em viveiros.

ABSTRACT: Enhanced efficiency potassium fertilizers can be a management tool that is crucial to crop sustainability in maize (Zea mays L.). However, there is a need for studies aimed at validating the use of these fertilizers in different production environments. This study aimed to evaluate the performance of maize under sources and rates of K through conventional and enhanced efficiency fertilizers in soil with high available K content. The experiment was carried out for two years in an Oxisol (605 g kg–1 of clay) with high K content (6.7 mmolc dm–3). Three sources were used, one conventional (KCl), one obtained by additives sprayed on the fertilizer surface (KCl-C), and one obtained by compacting KCl powder and adding additives (KCl-CC), associated with three K2O rates as top-dressing (50, 100, 150 kg ha–1) and a control without K2O. In all treatments, 48 kg ha–1 of K2O was applied in the sowing furrow. In the first year, maize yield increased linearly for both the KCl and KCl-C sources. The maximum yield (7,967 kg ha–1) for the KCl-CC was obtained at 88 kg ha–1. In the second year, the maximum yields for the KCl (7,553 kg ha–1) and KCl-C (8,166 kg ha–1) were obtained with 20 and 67 kg ha–1 K2O, respectively, while for the KCl-CC maize yield did not change. Enhanced efficiency K sources promote increases in maize yield ranging from 4.3 % to 7.1 %. Top-dressing K fertilization in high-fertility soils is a viable alternative for producers focused on increasing maize yield, mainly when enhanced efficiency sources are used.

Abstract The aim of this study was to evaluate the effects of the inclusion of palm oil on the ruminal environment and nutrient digestibility of sheep diets. Twenty rumen-cannulated sheep were kept in individual stalls equipped with feeding and drinking troughs The animals were fed five diets based on Elephant grass (Pennisetum purpureum Schum. cv. Roxo) silage and supplemented with 0, 25, 50, 75, or 100 g kg-1 of palm oil (based on total DM). The Elephant grass was harvested at 90 days of regrowth and the concentrate was based on ground corn grain, soybean meal and mineral mix (20 g kg-1 DM), offered to the sheep at a ratio of 1.5 g kg-1d-1 of body weight (restricted intake) to maintain a forage-to-concentrate ratio of 1:1, based on DM. There were no differences (P = 0.324) in ruminal disappearance and degradability parameters with up to 75 g of oil per kg of DM. Organic matter showed a linear reduction in apparent digestibility, while ether extract increased linearly. Palm oil affected the digestibility and nutritional parameters in ruminant diets.

ABSTRACT Objective: To determine whether polymorphisms of the IL10 and IL17 genes are associated with severe asthma control and bronchodilator reversibility in children and adolescents with severe asthma. Methods: This was a cross-sectional study, nested within a prospective cohort study of patients with severe asthma. Two outcomes were evaluated: asthma control and bronchodilator reversibility. We extracted DNA from peripheral blood and genotyped three single nucleotide polymorphisms: rs3819024 and rs2275913 in the IL17A gene; and rs3024498 in the IL10 gene. For the association analyses, we performed logistic regression in three genetic models (allelic, additive, and dominant). Results: The rs3024498 C allele in the IL10 gene was associated with failure to achieve asthma control despite regular treatment (p = 0.02). However, the G allele of the IL17A rs3819024 polymorphism was associated with failure to respond to stimulation with a b2 agonist. The rs2275913 polymorphism of the IL17A gene showed no relationship with asthma control or bronchodilator reversibility. Conclusions: In pediatric patients with severe asthma, the IL10 polymorphism appears to be associated with failure to achieve clinical control, whereas the IL17A polymorphism appears to be associated with a worse bronchodilator response. Knowledge of the involvement of these polymorphisms opens future directions for pharmacogenetic studies and for the implementation of individualized therapeutic management of severe asthma in pediatric patients.

RESUMO Objetivo: Determinar se existe relação entre polimorfismos dos genes IL10 e IL17 e controle da asma grave e reversibilidade com broncodilatador em crianças e adolescentes com asma grave. Métodos: Estudo transversal, aninhado em um estudo prospectivo de coorte com pacientes com asma grave. Foram avaliados dois desfechos: controle da asma e reversibilidade com broncodilatador. Extraímos DNA do sangue periférico e genotipamos três polimorfismos de nucleotídeo único: rs3819024 e rs2275913 no gene IL17A e rs3024498 no gene IL10. Para as análises de associação, realizamos regressão logística em três modelos genéticos (alélico, aditivo e dominante). Resultados: O alelo C do polimorfismo rs3024498 do gene IL10 apresentou relação com asma que permaneceu descontrolada mesmo com tratamento regular (p = 0,02). No entanto, o alelo G do polimorfismo rs3819024 do gene IL17A apresentou relação com ausência de resposta ao estímulo com b2-agonista. O polimorfismo rs2275913 do gene IL17A não apresentou relação com controle da asma ou reversibilidade com broncodilatador. Conclusões: Em pacientes pediátricos com asma grave, o polimorfismo do gene IL10 parece estar relacionado com ausência de controle clínico, ao passo que o polimorfismo do gene IL17A parece estar relacionado com pior resposta ao broncodilatador. O conhecimento a respeito do envolvimento desses polimorfismos abre perspectivas futuras para estudos farmacogenéticos e para a implantação de manejo terapêutico individualizado da asma grave em pacientes pediátricos.

Abstract Brazil is among the countries that most use pesticides in the world. These chemicals cause undesirable changes in ecosystems, particularly the contamination of non-target native forest species through drift. The nuisances caused by pesticides go beyond environmental damage and include public health problems. The objective of this work was to evaluate the effects of glyphosate on leaf gas exchange, photosynthetic pigments and morphoanatomy of seedlings of Eugenia dysenterica. The visual toxicity, physiological and morphoanatomical characteristics of E. dysenterica, when exposed to concentrations of 0, 550, 1110 and 2220 g a.e. ha-1 of glyphosate, were analyzed. The results indicate that the herbicide caused toxicity in the leaves in all treatments. Reductions in photosynthesis (A), stomatal conductance (gs), and transpiration (E) at 47 DAA, were also identified. Glyphosate caused damage to the anatomical structures of E. dysenterica leaves. From the data analyzed it is possible to affirm that plants of E. dysenterica are sensitive to the action of glyphosate. Visible symptoms such as chlorosis and necrosis in the leaf edge are indicators that can be used by rural communities as a warning of the risk of contamination.

Resumo O Brasil está entre os países que mais utiliza agrotóxicos no mundo. Essas substâncias químicas provocam modificações indesejáveis nos ecossistemas, destacando a contaminação de espécies florestais nativas não alvo, através da deriva. Os transtornos causados pelos agrotóxicos extrapolam os danos ambientais, sendo considerados problema de saúde pública. O objetivo deste trabalho foi avaliar os efeitos do glifosato nas trocas gasosas foliares, pigmentos fotossintéticos e morfoanatomia de plântulas de Eugenia dysenterica. Foram analisadas a toxicidade visual, as características fisiológicas e morfoanatômicas de E. dysenterica, expostas a concentrações de 0, 550, 1110 e 2220 g a.e. ha-1 do glifosato. Os resultados apontam que o herbicida causou toxicidade nas folhas em todos os tratamentos. Redução da fotossíntese (A), condutância estomática (gs) e da transpiração (E) aos 47 DAA, também foram identificados. O glifosato causou danos às estruturas anatômicas das folhas de E. dysenterica. Diante dos dados analisados é possível afirmar que plantas de E. dysenterica são sensíveis a ação do glifosato. Sintomas visíveis como clorose e necrose na borda foliar são indicadores que podem ser utilizados pelas comunidades rurais como alerta do risco de contaminação.

ABSTRACT Water stress impairs plant growth, resulting in the death of the plant in extreme cases. In vitro studies on stress-tolerant species can serve as the basis for improvement through genetic modifications aimed at minimizing damage and providing a controlled environment for performing biochemical and physiological assessments of plants under stress. The Velloziaceae family includes desiccation-tolerant and fire-resistant species; thus, these species can be used for analyzing protective mechanisms and reproductive responses to stress. We hypothesized that species with adaptability to survival under extremely dry conditions would respond to in vitro water stress through resprouting. This study evaluated the extent of water stress induced by the addition of sucrose, mannitol, or polyethylene glycol, the role of water stress as a trigger for in vitro multiplication, and its effect on biochemical responses in Vellozia jolyi, Vellozia punctulata, Vellozia pyrantha, and Vellozia seubertiana. Independent experiments were conducted by supplementing the following concentrations of sucrose, mannitol, and polyethylene glycol to the MS culture medium: sucrose (30 g L−1 [control], 75 g L−1, and 120 g L−1) and mannitol (0.0 g L−1 and 15.96 g L−1) for V. pyrantha; sucrose (15 g L−1 [control], 45 g L−1, and 60 g L−1) and mannitol (0.0 g L−1 and 7.9 g L−1) for the other species; and polyethylene glycol (50 g L−1, 100 g L−1, and 150 g L−1). When sucrose and mannitol were added, shoots had grown in all species, showing significant differences between treatments only for V. pyrantha. Polyethylene glycol did not induce shoot growth but, instead, diminished plant survival. The highest concentration of polyethylene glycol increased proline levels in V. pyrantha. All four species were resistant to water stress, owing to their ability to survive and reproduce under high concentrations of osmoregulators. Our study provides evidence that proline acts as an osmoprotectant of V. pyrantha.

RESUMO O estresse hídrico prejudica o crescimento das plantas, causando a morte em casos extremos. Estudos in vitro com espécies tolerantes ao estresse podem ser a base para o melhoramento genético, visando minimizar esses danos, além de fornecer um ambiente controlado para avaliações bioquímicas e fisiológicas de plantas sob estresse. A família Velloziaceae possui espécies tolerantes à dessecação e resistentes ao fogo, tornando-se um material valioso para a análise de mecanismos de proteção e resposta reprodutiva ao estresse. O objetivo foi avaliar o estresse hídrico com sacarose, manitol e polietilenoglicol como gatilho para multiplicação in vitro e seu efeito na resposta bioquímica de Vellozia jolyi, Vellozia punctulata Vellozia pyrantha e Vellozia seubertiana. Experimentos independentes foram realizados com concentrações de sacarose (30 controle; 75; 120 g L-1) + manitol (0.0; 15.96 g L-1) para V. pyrantha; sacarose (15 controle, 45 e 60 g L-1) + manitol (0.0; 7.9 g L-1) nas demais espécies e polietilenoglicol (0,0, 50, 100 e 150 g L-1) ambos em meio de cultura MS. No teste com sacarose + manitol, foram observadas brotações em todas as espécies com diferença estatística entre os tratamentos apenas para V. pyrantha. O polietilenoglicol não influenciou a indução de brotações e interferiu na porcentagem de sobrevivência de todas as espécies. A maior concentração de polietilenoglicol aumentou o teor de prolina de V. pyrantha. As quatro espécies são resistentes ao estresse hídrico, devido à sua capacidade de sobreviver e se reproduzir em altas concentrações de osmorreguladores. Há evidências que a prolina é um dos osmoprotetores de V. pyrantha.

RESUMO Objetivou-se quantificar os teores de C nos reservatórios da biomassa vegetal de diferentes espécies da Caatinga, floresta tropical seca do Nordeste do Brasil. Foi realizado um estudo florístico e fitossociológico prévio e selecionadas nove espécies de maior densidade absoluta, que representou 87% do fragmento. Foram amostradas biomassa dos compartimentos folhas e lenho de cada uma das espécies para determinação do teor de C. Como resultado, houve diferença nos teores foliares de C entre as espécies. O teor de C foliar das espécies variou entre 448,0 e 454,3 g C por kg de biomassa seca e o teor de C do lenho das espécies variou entre 451,4 e 453,1 g C por kg de biomassa seca. Esses resultados sugerem a utilização de fator de conversão de biomassa para carbono específico por espécie em Caatinga, como alternativa ao fator genérico de 50% recomendado pelo IPCC. Esta pesquisa revela que o uso do fator recomendado pelo IPCC superestima estoques de C em Caatinga. Mais estudos são encorajados para confirmar esse resultado.

ABSTRACT This study aimed to quantify the carbon contents in biomass reservoirs of different species in a Tropical Dry Forest located in Northeast, Brazil. We developed a previous floristic and phytosociological study and selected the nine highest absolute density (AD) species of the fragment, representing 87% of the fragment AD. We sampled the leaves and stem tissues of each species for carbon content determination. As result, the leaf C content differed between the species. The C leaf content ranged between 448.0 and 454.3 g C per kg of dry biomass. The C stem content varied between the species from 451.4 to 453.1 g C per kg of dry biomass. Our results suggest the use of a species-specific biomass-carbon conversion factor in Caatinga, as an alternative to the 50% factor recommended by the IPCC. This research reveals that the use of the factor recommended by the IPCC overestimates C stocks in Caatinga. Further studies are encouraged to confirm this result.

Abstract Germination increases total phenolic compounds (TF) concentration and antioxidant activity (AOx). The characterization and quantification of TPC, total flavonoids content (TPC), and AOx of beans (Phaseolus vulgaris) and peas (Pisum sativum) sprouts, germinated for ten days was performed. Results showed that the highest concentration of TPC for both sprouts was in days 6 and 7 (685.21 and 910.69 mg GAE/100 g dry matter) and TF varied only for beans. AOx of the pea sprouts was 512.64 and 6083.55 mg ET/100 g dry matter to DPPH and ORAC method, respectively, is higher than for bean. Regarding FRAP, bean sprouts showed better values (421.07 mg ET/100 g dry matter) compared to pea; in the CUPRAC analysis, sprouted bean showed better activity than a pea (85.76 and 44.05% inhibition). Germination induced variations in gallic and syringic acids in pea sprouts and of catechin and quercetin in the bean. Germination time and legume type are important factors the biological activity of sprouts.

RESUMO: Este artigo analisou a concentração regional da produção de lenha nativa do Rio Grande do Norte - Brasil, no período de 1990 a 2017. As informações da lenha nativa foram obtidas da produção da extração vegetal e da silvicultura, disponíveis no Instituto Brasileiro de Geografia e Estatística (IBGE). Analisou a conjuntura, a distribuição espacial da produção de lenha estadual por meio os quartis e mensurou a concentração por meio dos indicadores: Coeficiente de Gini (G), Índice de Concentração Compreensiva (CCI), Índice de Herfindahl-Hirschman (HHI) e Razão de Concentração [CR(k)]. Os resultados mostraram que houve decréscimo de -2,76% a.a. na produção de lenha nativa estadual, partindo de 5.280 x10³ m³ (1990) para 777 x10³ m³ (2017). O quartil municipal revelou que a maioria dos municípios produz pouca lenha; apesar do G ter inferido uma desigualdade muito forte a absoluta para os municípios produtores de lenha e fraca a nula para as mesorregiões, já o CCI mostrou para os municípios que é não concentrado e as mesorregiões tem concentração regional; HHI corroborou com esta afirmação mostrando um mercado altamente competitivo para os municípios e microrregiões e concentrado para as mesorregiões produtoras de lenha; o CR(k) dos quatro maiores municípios foi constatada uma concentração moderadamente baixa. Conclui-se que a lenha nativa do estado do Rio Grande do Norte possui estrutura de mercado competitiva.

ABSTRACT: This paperexamined the regional concentration of native fuelwood production in Rio Grande do Norte, Brazil, between 1990-2017. Information on native fuelwood was gathered fromforestry activitiescollected by the Brazilian Institute of Geography and Statistics (IBGE). This studyanalyzed the current situation and the spatial distribution of the state’s fuelwood production by quartiles. The following indicators were used to measure market concentration: Gini Coefficient (G), Comprehensive Concentration Index (CCI), Herfindahl-Hirschman Index (HHI) and Concentration Ratio [CR(k)]. In Rio Grande do Norte, there was a -2.76% annual decrease in the production of native fuelwood, from 5,280 x10³ m³ (1990) to 777 x10³ m³ (2017). Classification of the municipalities by quartile revealed that most municipalities had low fuelwood production. The G inferred a very strong to absolute inequality for the municipalities and a weak to null inequality for the mesoregions.The CCI demonstrated no market concentration in the municipalities and a regional concentration in the mesoregions. The HHI corroborated the CCI by affirming the presence of a competitive market for the municipalities and microregions and a concentrated market in the mesoregions.The CR(k) of the four largest municipalities indicated a moderately low concentration. This study concluded that there is a competitive market structure for native fuelwood inthe state of Rio Grande do Norte.

ABSTRACT The objective of this study was to evaluate the effects of dietary supplementation of purified lignin on the performance, relative organ weights, serum metabolites, and gene expression profiles of broiler chickens subjected to cyclic heat stress (HS). At 22 days old, 280 broilers were distributed in a completely randomized design with four treatments, ten repetitions, and seven birds per experimental unit. The birds were subjected to daily cyclic HS. A high temperature of 32±1 °C was maintained for 10 h/day (08:00–18:00 h), while a temperature of 22±1 °C was maintained for the remaining time. Treatments were a basal diet or basal diet with the addition of 5, 10, or 15 g of purified lignin/kg of diet. Data were analyzed using one-way ANOVA and means were compared by Tukey's test at 0.05 significance. There was no effect of lignin supplementation on performance, carcass yield, relative weights of the bursa, spleen, and liver, or serum levels of glucose, triglycerides, uric acid, malondialdehyde, triiodothyronine, or tetraiodothyronine. The abundance of mRNA of heat shock protein 70, nuclear factor-κB, glutathione peroxidase, and Cu,Zn-superoxide dismutase in the liver was similarly unaffected by treatments. Purified lignin supplementation does not improve performance or the antioxidant response of broiler chickens subjected to HS.

Abstract Saccharomyces cerevisiae (S. cerevisiae) has been studied as a model microorganism to understand the mechanism of glutathione (GSH) production. Furthermore, magnetic field (MF) application is a novel approach to stimulate microorganism metabolism to increase growth and produce more biomolecules. This study aimed to evaluate whether GSH production by S. cerevisiae ATCC 7754 would be affected by different pressure levels and MF application in an airlift bioreactor. Influence of pressure (0.0 - 0.15 MPa), medium recycle velocity (3.0 and 15.0 cm s-1) and MF application (0.0 and 3.0 mT) was evaluated. Biomass and GSH concentrations at high pressure and medium recycle velocity were higher than the ones in the control, whereas MF decreased biomass and GSH concentrations. The best results of biomass concentration and GSH production were 17.79 g L-1 and 162.92 mg L-1, respectively. at 0.05 MPa, 15.0 cm s- 1 and without any MF application. Results of the pressured airlift bioreactor showed that this kind of reactor may be used for producing GSH and for reducing energy costs.

Resumo O bioma Cerrado brasileiro apresenta em uma grande variedade de espécies endêmicas com diversos compostos bioativos, e Anadenanthera peregrina (L.) Speg é uma espécie promissora. Neste estudo, objetivamos realizar a caracterização fitoquímica e avaliar as atividades antioxidantes e antibacterianas contra Staphylococcus aureus e Escherichia coli do extrato hidroetanólico de cascas do caule de A. peregrina. As cascas foram coletadas no Jardim Botânico de Goiânia, Brasil. O extrato hidroetanólico foi obtido por percolação e submetido a triagem físico-química, estimativa de conteúdo fenólico total, impressão digital por cromatografia líquida de alta eficiência (HPLC) e determinação da atividade antioxidante (valores de IC50 foram calculados para o ensaio 2,2-difenil-1-picril-hidrazil) e antibacteriana. O pH do extrato foi de 5,21 e a densidade foi de 0,956 g/cm3. A triagem fitoquímica indicou a presença de glicosídeos cardíacos, ácidos orgânicos, açúcares redutores, saponinas hemolíticas, fenóis, cumarinas, taninos condensados, flavonóides, catequinas, depsídios e depsidonas derivados de benzoquinonas. O extrato mostrou intensa atividade hemolítica. O conteúdo fenólico total foi de 6,40 g de GAE 100 g-1. A análise por impressão digital por HPLC revelou a presença de ácido gálico, catequina e epicatequina. Confirmamos a atividade antioxidante do extrato. Além disso, o extrato não inibiu o crescimento de colônias de E. coli em nenhum volume testado, mas houve halos em torno das colônias de S. aureus nos três volumes testados. Estes resultados contribuem para uma melhor compreensão da composição química da casca de A. peregrina e apoia ainda mais as aplicações medicinais desta espécie.

Abstract The Brazilian Cerrado biome consists of a great variety of endemic species with several bioactive compounds, and Anadenanthera peregrina (L.) Speg is a promising species. In this study, we aimed to perform phytochemical characterization and evaluate the antioxidant and antibacterial activities against Staphylococcus aureus and Escherichia coli of the hydroethanolic extract of A. peregrina stem bark. The barks were collected in the Botanical Garden of Goiânia, Brazil. The hydroethanolic extract was obtained by percolation and subjected to physicochemical screening, total phenolic content estimation, high-performance liquid chromatography (HPLC) fingerprinting, and antioxidant (IC50 values were calculated for the 2,2-diphenyl-1-picrylhydrazyl assay - DPPH) and antibacterial activity determination. The pH of the extract was 5.21 and density was 0.956 g/cm3. The phytochemical screening indicated the presence of cardiac glycosides, organic acids, reducing sugars, hemolytic saponins, phenols, coumarins, condensed tannins, flavonoids, catechins, depsides, and depsidones derived from benzoquinones. The extract showed intense hemolytic activity. The total phenolic content was 6.40 g GAE 100 g-1. The HPLC fingerprinting analysis revealed the presence of gallic acid, catechin, and epicatechin. We confirmed the antioxidant activity of the extract. Furthermore, the extract did not inhibit the growth of E. coli colonies at any volume tested, but there were halos around S. aureus colonies at all three volumes tested. These results contribute to a better understanding of the chemical composition of A. peregrina stem bark and further support the medicinal applications of this species.